The isolation and the structure of glycolipids from Nakahara-Fukuoka sarcoma tissue is described. Glycolipids were purified by column chromatography on silicic acid, on Florisil and by crystallization with methanol. Four types of sphingolipid, ceramides, CMH, CDH and CTH were isolated in a pure state. In contrast to BP3/C3H mouse ascites sarcoma cells, N.F. sarcoma tissues contained nearly equimolar amounts of each glycolipid with a trace of globosides. The glycolipids were cleaved by various methods and the components were determined by G.L.C. mainly. CMH consisted of about 86% glucocerebrosides and about 14% galactocerebrosides. CDH was mainly lactosyl ceramide. CTH was mainly galactosyl (αl→4)galactosal (β1→4) glucosyl(1→1′) ceramide. The long chain bases present were 18-dihydrosphingosine and 18-sphingosine in the ratio of about 4:6 and the pattern of fatty acid was similar to that of sphingomeyelin.(1)