Platelet Coagulation Factor XI a -Inhibitor, a Form of Alzheimer Amyloid Precursor Protein
- 1 June 1990
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 248 (4959), 1126-1128
- https://doi.org/10.1126/science.2111585
Abstract
An inhibitor of coagulation factor XIa was purified from serum-free conditioned medium of HepG2 liver cells. Platelets stimulated with thrombin or calcium ionophore (A23187) secrete a protein functionally and immunologically identical to the inhibitor, implying a role for this inhibitor in hemostasis. Analysis of the amino-terminal amino acid sequence and immunologic reactivity showed the inhibitor to be a truncated form of the Alzheimer's amyloid precursor protein that contains a Kunitz-type serine protease inhibitor domain and at least a portion of the amyloid beta protein. It inhibits factor XIa and trypsin with a Ki of 450 +/- 50 pM and 20 +/- 10 pM, respectively. Heparin (1 unit/ml) did not significantly effect inhibition of trypsin, but inhibition of XIa was 15 times greater (Ki = 25 +/- 15 pM) in the presence of heparin.This publication has 28 references indexed in Scilit:
- Soluble derivatives of the β amyloid protein precursor of Alzheimer's disease are labeled by antisera to the β amyloid proteinBiochemical and Biophysical Research Communications, 1989
- Identification, biogenesis, and localization of precursors of Alzheimer's disease A4 amyloid proteinCell, 1989
- Novel precursor of Alzheimer's disease amyloid protein shows protease inhibitory activityNature, 1988
- A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitorsNature, 1988
- Regulation of factor XIa activity by platelets and alpha 1-protease inhibitor.JCI Insight, 1987
- The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptorNature, 1987
- Monoclonal anti-human factor VII antibodies. Detection in plasma of a second protein antigenically and genetically related to factor VII.JCI Insight, 1985
- Multiple intermediates in the reaction of bovine β‐trypsin with bovine pancreatic trypsin inhibitor (kunitz)Biopolymers, 1983
- Induction of the Platelet Release Reaction by PhytohemagglutininJCI Insight, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970