Application of flow cytometric methods for the routine detection of Cryptosporidium and Giardia in water
Open Access
- 1 May 1994
- Vol. 16 (1), 1-6
- https://doi.org/10.1002/cyto.990160102
Abstract
Cryptosporidium and Giardia are common causes of waterborne disease. The currently used methods of detecting these organisms in water rely on filtration capture, immunofluorescence labelling, and epifluorescence microscopy. These methods are inefficient, labour intensive, and require a highly skilled microscopist. We describe an alternative technique using flocculation concentration, followed by flow cytometry with fluorescence activated cell sorting. Environmental samples were analysed, and protozoan-like particles were sorted and collected before confirmation with epifluorescence microscopy. The technique was found to be significantly more sensitive and considerably faster than the conventional methods.Keywords
This publication has 27 references indexed in Scilit:
- A new method for the concentration of Cryptosporidium oocysts from waterJournal of Applied Bacteriology, 1993
- Taking the eye strain out of environmental cryptosporidium analysisLetters in Applied Microbiology, 1991
- CryptosporidiosisClinical Microbiology Reviews, 1991
- Isolation and Identification of Cryptosporidium from WaterWater Science & Technology, 1991
- Thames Water's Experiences with CryptosporidiumWater Science & Technology, 1991
- Giardia Cyst Concentrations in River WaterJournal AWWA, 1989
- Correlations of the Protozoa, Cryptosporidium and Giardia, with Water Quality Variables in a WatershedWater Science & Technology, 1988
- Occurrence and Significance of Cryptosporidium in WaterJournal AWWA, 1988
- Determination of Giardia muris Cyst Viability by Differential Interference Contrast, Phase, or Brightfield MicroscopyJournal of Parasitology, 1987