Contribution of immunoglobulin heavy-chain variable-region genes to antibody diversity
- 1 March 1980
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 284 (5753), 238-243
- https://doi.org/10.1038/284238a0
Abstract
A mouse cloned c[complementary]DNA probe containing a variable (V) region belonging to the VHIII subgroup was used in filter hybridizations to estimate the number of H chain V-genes in this subgroup of mouse and human DNA. There seem to be about 10 and 20 VH-genes hybridizing to this probe in mouse and human DNA, respectively. Studies of cross-hybridization of the related V.kappa.-genes from MOPC-21 and MPC-11 myelomas indicate that the experiments detect all members of the VHIII subgroup.This publication has 28 references indexed in Scilit:
- A κ-immunoglobulin gene is formed by site-specific recombination without further somatic mutationNature, 1979
- Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene.Proceedings of the National Academy of Sciences, 1979
- Sequences at the somatic recombination sites of immunoglobulin light-chain genesNature, 1979
- The arrangement and rearrangement of antibody genesNature, 1978
- Sequences of mouse immunoglobulin light chain genes before and after somatic changesCell, 1978
- A complete immunoglobulin gene is created by somatic recombinationCell, 1978
- Multiple related immunoglobulin variable-region genes identified by cloning and sequence analysis.Proceedings of the National Academy of Sciences, 1978
- Evidence for noncontiguous variable and constant region genes in both germ line and myeloma DNACell, 1978
- Evidence for somatic rearrangement of immunoglobulin genes coding for variable and constant regions.Proceedings of the National Academy of Sciences, 1976
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975