ACID PHOSPHATASE LOCALIZATION IN RABBIT EOSINOPHILS
Open Access
- 1 September 1967
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 34 (3), 745-756
- https://doi.org/10.1083/jcb.34.3.745
Abstract
Eosinophil (and heterophil) leukocytes of glycogen-induced rabbit peritoneal exudates were fixed for 1½ min in 2% glutaraldehyde and examined for acid phosphatase activity both biochemically and cytochemically. Biochemical assays showed that enzymatic activity had been inhibited by only ∼10% under these conditions. The cytochemical reaction in the eosinophil was confined to the granules in which the reaction product appeared in the matrix, not in the crystalline core (or in the core region after the latter's extraction). Granules wherein the matrix was disrupted and the crystalline core degraded or extracted showed the most intense deposition of reaction product, whereas well preserved granules with morphologically intact matrix and crystals were unreactive. Yet, not all disrupted granules gave a positive reaction, indicating that disruption was a necessary but not sufficient condition for reactivity. In many eosinophil leukocytes, most if not all granules were acid phosphatase-positive, provided they had become disrupted to a certain degree. Factors possibly involved in converting the granules from an unreactive to a reactive state are discussed.This publication has 19 references indexed in Scilit:
- Light and Electron Microscopic Localization of Acid Phosphatase Activity in Human EosinophilsAmerican Journal of Clinical Pathology, 1966
- ORIGIN OF GRANULES IN POLYMORPHONUCLEAR LEUKOCYTESThe Journal of cell biology, 1966
- CELL JUNCTIONS IN AMPHIBIAN SKINThe Journal of cell biology, 1965
- The surface activity of tranquilizersBiochemical Pharmacology, 1963
- ISOLATION OF GRANULES FROM EOSINOPHIL LEUCOCYTES AND STUDY OF THEIR ENZYME CONTENTThe Journal of Experimental Medicine, 1963
- Electron Microscopy of Human White Blood Cells and Their Stem CellsInternational Review of Cytology, 1962
- SIMPLE METHODS FOR "STAINING WITH LEAD" AT HIGH pH IN ELECTRON MICROSCOPYThe Journal of cell biology, 1961
- THE ISOLATION AND PROPERTIES OF THE SPECIFIC CYTOPLASMIC GRANULES OF RABBIT POLYMORPHONUCLEAR LEUCOCYTESThe Journal of Experimental Medicine, 1960
- INTERACTIONS BETWEEN RABBIT POLYMORPHONUCLEAR LEUCOCYTES AND STAPHYLOCOCCIThe Journal of Experimental Medicine, 1959
- A STUDY OF FIXATION FOR ELECTRON MICROSCOPYThe Journal of Experimental Medicine, 1952