Interactions of Gonadotropins with Corpus Luteum Membranes. III. Electron Microscopic Localization of [125I]-hCG Binding to Sensitive and Desensitized Ovaries Seven Days after PMSG-hCG1
- 1 March 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 20 (2), 362-376
- https://doi.org/10.1095/biolreprod20.2.362
Abstract
Transmission electron micrographs, with and without prior infusion of horseradish peroxidase, and scanning electron microscopy of fractured corpora lutea demonstrated extensive microvillus development on the surface of rat luteal cells 7 days after priming with PMSG and hCG. These microvilli were primarily on the capillary face of the luteal cells, while the basolateral faces of the cell were largely composed of smooth plasma membrane with some gap junctions between adjoining cells, Injection of a small dose of [1 2 5I]-hCG revealed localization of silver grains primarily on the microvillus surfaces and, to lesser extents, on the basolateral plasma membrane and in subplasma membrane cytosolic vesicles. Pinocytosis was clearly demonstrated. Injection of a dose of [1 2 5I]-hCG sufficient to desensitize the hCG stimulatable adenylate cyclase and to result in extensive loss of hCG receptors did not enhance the transfer of silver grains into cytoplasmic vesicles. This dose of 1 2 5I]-hCG decreased the area occupied by microvilli and increased the area of basolateral membrane.This publication has 11 references indexed in Scilit:
- Interactions of Gonadotropins with Corpus Luteum Membranes. I. Properties and Distributions of Some Marker Enzyme Activities after Subcellular Fractionation of the Superovulated Rat Ovary*Endocrinology, 1978
- Proteolytic enzyme activation of rat ovarian adenylate cyclaseProceedings of the National Academy of Sciences, 1977
- The Porcine Ovarian Follicle. II. Electron Microscopic Study of Surface Features of Granulosa Cells at Different Stages of Development1Biology of Reproduction, 1977
- Gonadotropin-induced regulation of luteinizing hormone receptors and desensitization of testicular 3':5'-cyclic AMP and testosterone responses.Proceedings of the National Academy of Sciences, 1977
- LOCALIZATION OF GONADOTROPIC HORMONES IN LYSOSOMES OF OVINE LUTEAL CELLS1977
- Gonadotropin-induced loss of hormone receptors and desensitization of adenylate cyclase in the ovary.Journal of Biological Chemistry, 1976
- Adenylyl Cyclase Activities in Ovarian Tissues. III. Regulation of Responsiveness to LH, FSH, and PGEX in the Prepubertal, Cycling, Pregnant, and Pseudopregnant RatEndocrinology, 1976
- Observations on the Ultrastructure of Luteal Cells from PMS and PMS-HCG Treated Immature RatsEndocrinology, 1966
- THF EARLY STAGES OF ABSORPTION OF INJECTED HORSERADISH PEROXIDASE IN THE PROXIMAL TUBULES OF MOUSE KIDNEY: ULTRASTRUCTURAL CYTOCHEMISTRY BY A NEW TECHNIQUEJournal of Histochemistry & Cytochemistry, 1966
- THE PREPARATION OF 131I-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITYBiochemical Journal, 1963