Effect of Sucrose Diet on Insulin Secretion In Vivo and In Vitro and on Triglyceride Storage and Mobilisation of the Heart of Rats

Abstract
Basal heart triacylglycerol (TG) (μmole triacylglycerol/g of dry weight) (- before “in vitro” Langendorff perfusion -) was significantly higher in animals rendered chronically hypertriglyceridaemic (H) by a 63% sucrose-rich diet than in controls (C, standard diet); 28 ± 2.6 X ± SEM vs. 19.3 ± 1.2; respectively (p < 0.01). After 40′ perfusion with Krebs-Henseleit buffer + 5.5 mM glucose, 2.5 mM Ca++, TG content fell to 14.2 ± 0.6 in C and 14.9 ± 1.9 in H (n.s.). Administration of 1 nmol × min-1 of glucagon (Gn) from min 20 to 40 reduced TG to 9.0 ± 0.5 in C (p < 0.05). In contrast no effect of Gn was observed in H (TG at min 40: 16.7 ± 2.5). Glycogen (Gly) content (μmol/g of dry weight) after Gn perfusion fell from 30 ± 1.9 to 17 ± 2.1 (p < 0.01) in C, while again no effect was recorded in H. “In vivo” plasma glucose fractional coefficient disappearance rate was lower (p < 0.001) in H: 1.01 × 10-2 ± 0.09 × 10-2 vs 2.61 × 10-2 ± 0.14 × 10-2 in C, in spite of H showing hyperinsulin secretion. Hyperinsulinism was further documented by “in vitro” Iri release studies from incubated pancreas pieces. In the absence of glucose (G) from the incubation medium H produced 541 ± 19.8 mU/mg weight tissue/20′, while C produced 91.2 ± 12.7 (p < 0.001). With 100 mg% G, H released 1058 ± 259 and C 377 ± 82.5 (p < 0.001). It is suggested that hyperinsulin secretion plus insulin resistance may account for the above findings.