One‐ and two‐dimensional electrophoresis in micro‐slab gels

Abstract

All acrylamide slab gel electrophoresis widely used for various separation problems at the marcro‐level can be converted readily to micro‐versions. This is demonstrated in isoelectric focusing, homogeneous or gradient micro‐slab gels, and two‐dimensional separations. The different types of micro‐slab gel electrophoresis have the same resolving capability as the corresponding macro‐method, which is demonstrated with different biological samples. Enzyme staining after two‐dimension separations is also possible. The apparatus, preparation and handling of the different micro‐slab gels is described in full detail.