Human c-myb protooncogene: nucleotide sequence of cDNA and organization of the genomic locus.

Abstract

We have isolated cDNA clones of the human c-myb mRNA that contain .apprxeq. 3.4 kilobases of the .apprxeq. 3.8-kilobase mRNA sequence. Nucleotide sequence analysis shows that the c-myb mRNA contains an open reading frame of 1920 nucleotides, which could encode a 72-kDa protein. The cDNA nucleotide sequence and the predicted amino acid sequence of the c-myb protein are highly homologous to the corresponding chicken and mouse proteins. In particular, a region toward the NH2 terminus of the protein containing a 3-fold tanden repeat of 51 residues is evolutionarily conserved and is the only region of homology with the Drosophila c-myb protein. This region may represent a functionally important structure, most likely the DNA-binding domain. cDNA clones have been used to isolate genomic clones and to define a preliminary intron/exon organization of the c-myb gene. Identification of 5'' and 3'' coding and noncoding exons indicates that the human c-myb locus spans a 40-kilobase region.