Phosphorylated Syntaxin 1 Is Localized to Discrete Domains Along a Subset of Axons
Open Access
- 15 June 2000
- journal article
- Published by Society for Neuroscience in Journal of Neuroscience
- Vol. 20 (12), 4535-4544
- https://doi.org/10.1523/jneurosci.20-12-04535.2000
Abstract
Syntaxin 1 is a SNARE protein that plays a central role in synaptic vesicle (SV) exocytosis. We generated an antibody that specifically recognizes a casein kinase II-mediated phosphorylation on serine-14 of syntaxin 1. In this report we show that this phosphorylation occursin vivoand is developmentally regulated in the rat brain, rising to a level of 40% of the total syntaxin in adult animals. Phosphorylated syntaxin is preferentially associated with SNAP-25 and localizes to discrete domains of the axonal plasma membrane that do not colocalize with pools of synaptic vesicles. These phosphosyntaxin domains may define fusion sites for a novel class of vesicles outside classical active zones.Keywords
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