The Effect of Methylation of the 6 Oxygen of Guanine on the Structure and Stability of Double Helical DNA
- 1 August 1985
- journal article
- research article
- Published by Taylor & Francis in Journal of Biomolecular Structure and Dynamics
- Vol. 3 (1), 57-66
- https://doi.org/10.1080/07391102.1985.10508398
Abstract
The effect of methylation of the 0–6 position of guanine in short segments of double helical DNA has been investigated by molecular mechanical simulations on the sequences d(CGCGCG)2, d(CGC+AFs-OMG+AF0-CG)2, d(CGT+AFs-OMG+AF0-CG)2, d(CGC+AFs-OMC+AF0-CG/(CGCGCG), d(CGC+AFs-OMG+AF0-CG/d(CGTGCG), d(CGCGAATTCGCG)2 and d(CGCGAATTC+AFs-OMG+AF0-CG)2. Guanines methylated at the 0–6 position are found to form hydrogen bonds of roughly equal strength to cytosine and thymine. The optimum structure of these modified base pairs are not dramatically different from normal GC pairs, but both involve some bifurcation of the proton donors of cytosine (4NH2) or thymine (3NH) between the guanine N3 and O6 groups.This publication has 9 references indexed in Scilit:
- An approach to computing electrostatic charges for moleculesJournal of Computational Chemistry, 1984
- A new force field for molecular mechanical simulation of nucleic acids and proteinsJournal of the American Chemical Society, 1984
- Specifically alkylated DNA fragments. Synthesis and Physical Characterization of D[cGC(o2Me)GCG]and d[cGT(O6Me) GCG]Nucleic Acids Research, 1983
- Kinetics of repair of O6-methylguanine in DNA by O6-methylguanine-DNA methyltransferase in, vitro and in, vivoBiochemical and Biophysical Research Communications, 1982
- Mechanism of activation of a human oncogeneNature, 1982
- AMBER: Assisted model building with energy refinement. A general program for modeling molecules and their interactionsJournal of Computational Chemistry, 1981
- DNA synthesis with methylated poly(dC-dG) templates. Evidence for a competitive nature to miscoding byBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1979
- Repair of O6-methylguanine in adapted Escherichia coli.Proceedings of the National Academy of Sciences, 1978
- The properties of
methylguanine in templates for RNA polymeraseBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973