Regulation of translation in rabbit reticulocytes and mouse L-cells; comparison of the effects of temperature
- 1 February 1976
- journal article
- research article
- Published by Wiley in Journal of Cellular Physiology
- Vol. 87 (2), 157-166
- https://doi.org/10.1002/jcp.1040870204
Abstract
Various parameters of protein synthesis were analyzed in rabbit reticulocytes exposed to various temperatures for up to five hours. Between 10°C and 40°C total protein synthesis exhibited two different apparent activation energies (36 kcal/mole, 10–24°C; 22 kcal/mole, 24–40°C), as did protein elongation and release (35 kcal/mole, 10–25°C; 12 kcal/mole, 25–40°C). However, the level of polysomes remained essentially unchanged between 0°C and 42°C which implies that the activation energy for polypeptide initiation is quite similar to that for elongation and is also biphasic. This situation is different from that in cultured mouse L-cells where the polysome level is dependent on temperatures. Nevertheless, reticulocytes and L-cells appear to be similar in their temperature dependence of initiation and in their rate of elongation (5–6 amino acids/second at 36°C).This publication has 13 references indexed in Scilit:
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