Identification of the bovine brain Ins(1,4,5)P3 5‐phosphatase after SDS‐polyacrylamide gel electrophoresis
- 5 June 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 249 (2), 321-323
- https://doi.org/10.1016/0014-5793(89)80650-7
Abstract
Ins(1,4,5)P3 5-phosphatase catalyses the dephosphorylation of Ins(1,4,5)P3 in the 5-position. In a high speed soluble fraction of bovine brain, there are two soluble 5-phosphatases: type I and type II. The purified Ins(1,4,5)P3 5-phosphatase type I exhibits a major silver-stained band of 43 kDa on denaturing (SDS) gels. It is possible to extract the 5-phosphatase activity form a duplicate lane after gel electrophoresis. The 43 kDa region contains the extractable Ins(1,4,5)P3 5-phosphatase activity.Keywords
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