Immunoglobulin Synthesis on Free and Bound Polyribosomes of Rabbit Lymph Node Cells

Abstract

Lymph node cells from immunized rabbits were pulse-labeled in vitro with ³Hleucine and fractionated on sucrose gradients to separate free and membrane-bound polyribosomes. Nascent polypeptides released from polyribosomes were precipitated with antiserum specific for rabbit immunoglobulin. The results indicate that immunoglobulin is synthesized preferentially by bound polyribosomes.