Involvement of Anti-Ig-Activated Serine Protease in the Generation of Cytoplasmic Factor(s) that Are Responsible for the Transmission of Ig-Receptor-Mediated Signals
- 1 October 1979
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 123 (4), 1504-1510
- https://doi.org/10.4049/jimmunol.123.4.1504
Abstract
Involvement of serine protease-activation in the generation of cytoplasmic factor(s) that induced NHP-specific protein kinase activity in nuclei in anti-Ig-stimulated cells was described. DFP or PMSF with anti-Ig inhibited the induction of cytoplasmic factor(s), whereas pretreatment of cells with DFP or PMSF without anti-Ig did not show any inhibitory effect on anti-Ig-induced generation of cytoplasmic factor(s). TAME or BAME with anti-Ig inhibited the generation of cytoplasmic factor(s) and the simultaneous addition of TAME or BAME with DFP protected the generation of cytoplasmic factor(s) against the inhibitory effect of DFP, showing the involvement of trypsin-like, arginine-type serine protease in anti-Ig-induced generation of cytoplasmic factor(s). Anti-Ig-stimulated membrane preparations induced cytoplasmic factor(s) in normal cytoplasm. The m.w. of precursor proteins present in resting B cells and active cytoplasmic factor(s) were approximately 150,000 and 45,000, respectively. These results showed that anti-Ig-activated membrane-bound serine protease split precursor proteins in resting B cells into active cytoplasmic factor(s) responsible for signal transmission.This publication has 10 references indexed in Scilit:
- Activation of mouse lymphocytes by anti-immunoglobulin. I. Parameters of the proliferative response.The Journal of Experimental Medicine, 1978
- ORGANOPHOSPHORUS INHIBITION OF LYSOSOMAL ENZYME-SECRETION FROM POLYMORPHONUCLEAR LEUKOCYTES - EVIDENCE OF A LACK OF A REQUIREMENT FOR ESTERASE ACTIVATION1978
- Induction and properties of cytoplasmic factor(s) which enhance nuclear nonhistone protein phosphorylation in lymphocytes stimulated by anti-Ig.The Journal of Experimental Medicine, 1977
- Biphasic Effect of Cyclic AMP on IgG Production and on the Changes of Non-Histone Nuclear Proteins Induced with Anti-Immunoglobulin and Enhancing Soluble FactorThe Journal of Immunology, 1977
- Activation of stimulus-specific serine esterases (proteases) in the initiation of platelet secretion. I. Demonstration with organophosphorus inhibitors.The Journal of Experimental Medicine, 1976
- The Requirement for Esterase Activation in the Anti-Immunoglobulin-Triggered Movement of B LymphocytesThe Journal of Immunology, 1976
- ANTI-IMMUNOGLOBULIN STIMULATION OF MURINE LYMPHOCYTES .1. AGE DEPENDENCY OF PROLIFERATIVE RESPONSE1976
- MECHANISMS OF THE INHIBITION OF CHEMOTAXIS BY PHOSPHONATE ESTERSThe Journal of Experimental Medicine, 1967
- MECHANISMS OF IMMUNOLOGIC INJURY OF RAT PERITONEAL MAST CELLSThe Journal of Experimental Medicine, 1966
- A simple method for the preparation of 32P-labelled adenosine triphosphate of high specific activityBiochemical Journal, 1964