A Comparison of New World Alphaviruses in the Western Equine Encephalomyelitis Complex by Immunochemical and Oligonucleotide Fingerprint Techniques
- 1 April 1980
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 47 (2), 261-282
- https://doi.org/10.1099/0022-1317-47-2-261
Abstract
We have examined the molecular basis for the observed antigenic differences between isolates of western equine encephalomyelitis (WEE) virus and those of a serologically related alphavirus from the eastern United States designated Highlands J (HJ). The structural proteins of WEE virus isolates have mol. wt. of 55 × 103 (E1), 47 × 103 (E2) and 33 × 103 for the nucleocapsid. The E1 glycoprotein had an isoelectric point (pI) of 6.4 and induced haemagglutination-inhibiting (HI) antibody which was specific for WEE virus. The E2 glycoprotein of WEE virus had a pI of 8.4 and induced antibody which was virus specific by neutralization (PRNT) but cross-reacted with HJ virus in the radioimmune precipitation (RIP) test. Envelope glycoproteins of HJ virus isolates had mol. wt. of 58 × 103 (E1) and 49 × 103 (E2) respectively. The E1 glycoprotein from HJ virus had a pI of 6.8 and induced antibody which reacted specifically in the HI, PRNT and RIP tests. Isolated E2 protein of HJ virus had a pI of 9.1 and induced antibodies which were reactive at equal titre with both WEE and HJ viruses by RIP.This publication has 22 references indexed in Scilit:
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