Effect of Insulin Pretreatment on Glucose and Lipid Metabolism of Liver Slices from Normal Rats.

Abstract

Summary Liver slices from normal fed rats were incubated in physiologic bicarbonate buffer, and the effect of increasing glucose concentration and insulin pretreatment on hepatic glucose metabolism was studied. Exposure of the liver slice to increased levels of glucose resulted in the recovery of more glucose-6¹⁴C in CO₂ relative to lipid pathways. Insulin pretreatment, in the presence of a “high” medium glucose concentration, resulted in a preferential incorporation of glucose-6¹⁴C into total lipid and triglyceride relative to CO₂ and into fatty acid to a greater extent than into glycerol. The effects of insulin were dependent upon the presence of a “high” concentration of glucose in the medium.