The Functional Expression of Recombinant Human Thyrotropin Receptors in Nonthyroidal Eukaryotic Cells Provides Evidence that Homologous Desensitization to Thyrotropin Stimulation Requires a Cell-Specific Factor*

Abstract

TSh desensitization involves decreased coupling of the TSH receptor to the adenylate cyclase regulatory protein Gs. There is evidence that a desensitization protein in thyroid cells plays a role in this process. The molecular cloning of the human TSH receptor and its stable expression in Chinese hamster ovary (CHO-TSHR) cells allowed us to test whether or not TSH desensitization can occur in an nonthyroidal cell. Similar to human thyroid cells, maximal stimulation of cAMP levels in CH-TSHR cells was attained after 30-60 min of exposure to bovine TSH. Unlike in human thyroid cells, however, preincubation of CHO-TSHR cells with TSH for 12-16 h did not decrease the subsequent cAMP response to a 1-h pulse of TSH stimulatoin. That is, the human TSH receptor in CHO-TSHR cells does not undergo functional desensitization. Scatchard plot analysis of specific TSH binding to the CHO-TSHR cells revealed high and low affinity sites (Ka of 1.8 .+-. 0.4 .times. 109 M-1 and 1.4 .+-. 0.3 .times. 107 M-1, respectively), with approximately 105 TSH receptors per cell. This is 10 to 100-fold greater than the number of TSH receptors estimated to be present on human thyroid cells. Untransfected CHO cells exhibited only the low affinity binding site. Prior exposure of CHO-TSHR cells to bovine TSH or to (Bu)2cAMP for periods up to 2 h did not reduce [125I]TSH binding to these cells. In summary, desensitization or the adenylate cyclase response to TSH stimulation does not ocur in nonthyroidal cells expressing a human TSH receptor with normal functional and TSH binding characteristics. These data support the concept that a cell-specific protein may be involved in homologous TSH desensitization.