Metabolism of d -Fructose in Aerobacter aerogenes: Analysis of Mutants Lacking d -Fructose 6-Phosphate Kinase and d -Fructose 1,6-Diphosphatase

Abstract

The relative significance of the pathways for the conversion of D-fructose to D-fructose 1,6-diphosphate via D-fructose 1-phosphate or D-fructose 6-phosphate in A. aerogenes PRL-R3 was assessed by observing growth patterns of mutants lacking either D-fructose 6-phosphate kinase or D-fructose 1,6-diphosphatase. The mutant lacking D-fructose 6-phosphate kinase grew well on D-fructose or glycerol but not on D-glucose, whereas the mutant lacking D-fructose 1,6-diphosphatase grew on D-glucose but not on D-fructose or glycerol. The data confirm that the pathway of D-fructose metabolism is primarily through D-fructose 1-phosphate rather than D-fructose 6-phosphate.