Differential synthesis of blocked and unblocked 5'-termini in reovirus mRNA: effect of pyrophosphate and pyrophosphatase.
- 1 October 1976
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (10), 3448-3452
- https://doi.org/10.1073/pnas.73.10.3448
Abstract
Viral mRNA synthesized in vitro by reovirus-associated enzymes contained 5''-terminal ppG, GpppG or m7GpppGm. Selective synthesis of 1 type of 5''-end was obtained by adjusting the composition of the transcription reaction mixture based on a series of reactions proposed for formation of blocked, methylated 5''-terminal sequences of mRNA (cap formation). The presence of PPi increased the proportion of ppG 5''-ends, while addition of pyrophosphatase [from yeast] yielded mRNA with 5''-terminal GpppG, which in the presence of a methyl donor was converted to m7GpppGm.This publication has 32 references indexed in Scilit:
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