Membrane-Associated ATPases in Isolated Secretory Vesicles
- 1 March 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 71 (3), 594-597
- https://doi.org/10.1104/pp.71.3.594
Abstract
Polysaccharide-containing vesicles were collected from secretory cells maintained in liquid culture. Characterization of membrane-associated nucleosidephosphatases revealed that the vesicles specifically hydrolyze ATP have a pH optimum between 6.0 and 6.5 and are stimulated by inorganic cations, especially K+. The ATPase activity in these vesicles was inhibited by orthovanadate and N,N''-dicyclohexylcarbodiimide; other inhibitors, such as oligomycin, sodium azide and diethylstilbestrol were generally ineffective. Apparently, vesicles derived from the Golgi apparatus have partially differentiated into plasmalemma before they fuse with the plasma membrane.This publication has 11 references indexed in Scilit:
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