Correlative Inhibition of Lateral Bud Growth inPhaseolus vulgarisL. Isolation of Indoleacetic Acid from the Inhibitory Region

Abstract

The young, growing leaves have been shown by excision treatments to be responsible for the correlative inhibition of lateral bud growth in Phaseolus vulgaris. The presence of indoleacetic acid in apical portions of the stem bearing young leaves has been demonstrated by extraction and collection in agar (‘agar diffusion’) followed by purification and analysis by bioassay, chromogenic assay, and gas chromatography-mass spectrometry. The relevance of these results to the possible involvement of indoleacetic acid in correlative phenomena is discussed