Antigen presentation to HLA class II-restricted measles virus-specific T-cell clones can occur in the absence of the invariant chain.
- 1 February 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (4), 1209-1212
- https://doi.org/10.1073/pnas.85.4.1209
Abstract
A human fibroblast line expressing HLA-DR1 antigen on its surface was generated by transfection with DR.alpha. and DR.beta. cDNAs. Expression of the invariant chain gene was not detectable in the transfected fibroblasts and was not induced by infection with measles virus. Lysis of measles virus-infected cells occurred with DR1- but not with DR4-restricted measles virus-specific cytotoxic T-lymphocyte (CTL) clones and was inhibited by a monoclonal antibody specific for DR antigen. Therefore, the invariant chain is not required for DR-restricted presentation of measles virus antigens by this fibroblasts line. Transfected fibroblasts were lysed as efficiently as an autologous B-cell line even though they expressed much less surface DR antigen. Lysis of both the transfected fibroblasts and the B-cell line was insensitive to treatment with chloroquine. These results demonstrate that expression of a DR .alpha..beta. heterodimer at the surface of this fibroblast line is necessary and sufficient for presentation of measles virus antigens to specific CTL clones.This publication has 27 references indexed in Scilit:
- Purified lymphocyte function-associated antigen 3 binds to CD2 and mediates T lymphocyte adhesion.The Journal of Experimental Medicine, 1987
- Efficient cell surface expression of class II MHC molecules in the absence of associated invariant chain.The Journal of Experimental Medicine, 1986
- Two antigen-independent adhesion pathways used by human cytotoxic T-cell clonesNature, 1986
- Immunology: The ins and outs of antigen processing and presentationsNature, 1986
- Differences in antigen presentation to MHC class I-and class II-restricted influenza virus-specific cytolytic T lymphocyte clones.The Journal of Experimental Medicine, 1986
- Chloroquine affects biosynthesis of Ia molecules by inhibiting dissociation of invariant (gamma) chains from alpha-beta dimers in B cells.The Journal of Experimental Medicine, 1985
- Impaired measles virus-specific cytotoxic T cell responses in multiple sclerosis.The Journal of Experimental Medicine, 1985
- Gene transfer of H-2 class II genes: Antigen presentation by mouse fibroblast and hamster B-cell linesCell, 1984
- The class II molecules of the human and murine major histocompatibility complexCell, 1984
- Expression of HLA-DR antigens at the surface of mouse L cells co-transfected with cloned human genesNature, 1983