Natural anti‐IgE auto‐antibodies interfere with diagnostic IgE determination
- 1 May 1990
- journal article
- research article
- Published by Wiley in Clinical and Experimental Allergy
- Vol. 20 (3), 295-309
- https://doi.org/10.1111/j.1365-2222.1990.tb02687.x
Abstract
A simple method is described which enables measurement of anti-IgE antibodies in free form as well as in immune complexes of IgE and anti-IgE. Anti-IgE antibodies were purified from serum of one selected blood donor with highly elevated levels of such autoantibodies. These purified anti-IgE auto-antibodies inhibited the measurement of myeloma IgE. Purification also revealed that 98% of the subject''s serum IgE was masked by anti-IgE auto-antibodies. Our data suggest that IgE determinations in sera containing anti-IgE antibodies might be underestimated.This publication has 22 references indexed in Scilit:
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