Theory of gel electrophoresis of DNA: Orientational effects
- 1 January 1996
- journal article
- Published by Wiley in Electrophoresis
- Vol. 17 (6), 1018-1026
- https://doi.org/10.1002/elps.1150170609
Abstract
Some aspects of the reptation model of gel electrophoresis of DNA, with emphasis on the orientational effects, are considered. A master equation for the reptational dynamics with the end boundary conditions is proposed. Based on this equation, the orientation and dynamics of the persistent‐chain are investigated. In particular, the role of trap conformations for this chain, which essentially reduce the chain mobility, was analyzed. A transient orientation of the chain during pulsed field gel electrophoresis is also discussed.Keywords
This publication has 21 references indexed in Scilit:
- Anomalous Dispersion in Gel Electrophoresis: A Solvable Biased Reptation ModelEurophysics Letters, 1988
- Quantitative analysis of the three regimes of DNA electrophoresis in agarose gelsBiopolymers, 1988
- Self-trapping and anomalous dispersion of DNA in electrophoresisPhysical Review Letters, 1987
- On the stretching of DNA in the reptation theories of gel electrophoresisBiopolymers, 1987
- On the reptation theory of gel electrophoresisBiopolymers, 1986
- New Biased-Reptation Model For Charged PolymersPhysical Review Letters, 1985
- Theory of gel electrophoresis of DNABiopolymers, 1985
- Mobility of DNA in gel electrophoresisBiopolymers, 1982
- Why does the electrophoretic mobility of DNA in gels vary with the length of the molecule?Biopolymers, 1982
- Reptation of a Polymer Chain in the Presence of Fixed ObstaclesThe Journal of Chemical Physics, 1971