Lipogenic Activity of a Potent Lipolytic Hormone: Sheep β Lipotropin (β-LPH) - II. Further Effects of Sheep β-LPH on Specifically Labeled Glucose and the Localization of Lipogenic Active Center of the Molecule

Abstract

A study of β-LPH on specifically labeled glucose metabolism by epididymal fat pads in vitro showed that β-LPH enhanced the conversion of both 1-¹⁴C and 6-¹⁴C glucose to CO₂ and fatty acids. The adipose tissue oxidized glucose-1-^I4C to CO₂ to a greater extent than glucose-6-¹⁴C and presence of β-LPH did not change this usual pattern. Studies with β_h- MSH and with five fragments of the β-LPH molecule obtained by cleavage with cyanogen bromide show that the center for lipogenic activity involves the sequence 48-65, but additional amino acids at either end of this core are necessary before noticeable lipogenic activity appears. A similar sequence is known to be required for lipolytic activity.