STEREOCHEMICAL ANALYSIS OF THE SPECIFICITY OF PANCREATIC RNASE WITH POLYFORMYCIN AS SUBSTRATE: DIFFERENTIATION OF THE TRANSPHOSPHORYLATION AND HYDROLYSIS REACTIONS
- 1 February 1969
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 62 (2), 581-588
- https://doi.org/10.1073/pnas.62.2.581
Abstract
A stereochemical analysis of the substrate and inhibitor specificities of bovine pancreatic ribonuclease A is presented. A scheme is proposed in which the binding specificity for this protein-nucleic acid interaction is rationalized in terms of a simple system of H-bonds. The functional groups that govern substrate binding for transphosphorylation and hydrolysis, respectively, are considered and differentiated, and predictions are offered concerning the interaction of presumptive substrates with RNase.Keywords
This publication has 11 references indexed in Scilit:
- Conformational properties of polyformycin: a polyribonucleotide with individual residues in the syn conformation.Proceedings of the National Academy of Sciences, 1968
- The Structure of Ribonuclease-S at 3.5 A ResolutionJournal of Biological Chemistry, 1967
- Nucleoside conformations: an analysis of steric barriers to rotation about the glycosidic bondJournal of Molecular Biology, 1967
- Zum Mechanismus der Ribonuclease‐ReaktionEuropean Journal of Biochemistry, 1967
- Optical Rotatory Dispersion of Nucleic Acid Derivatives. VIII. The Conformation of Pyrimidine Nucleosides in Solution*Biochemistry, 1967
- Polynucleotide analoguesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1966
- The polymerization of 8-Azaguanosine 5′-diphosphate by polynucleotide phosphorylaseBiochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 1962
- The Interaction between Ribonuclease and Mononucleotides as Measured SpectrophotometricallyJournal of Biological Chemistry, 1961
- Research on pancreatic ribonuclease. I. The inhibition of cyclic phospho-diesterase activity of bovine pancreatic ribonuclease by several substrate analogues.1961
- Base pairing in DNAJournal of Molecular Biology, 1960