Studies on sulphatases. 7. A preliminary account of the glycosulphatase of Littorina littorea

Abstract

Glycosulphatase was shown to be present in appreciable amounts in the visceral hump of the large periwinkle, Littorina littorea. A stable acetone-dried concentrate of the enzyme was made. Alkyl-, aryl- and chondrosulphatases and beta-glucuronidase are also present in the concentrate. The findings of Egami (Jour. Chem. Soc. Japan, 63. p. 763 1942) as to the impure nature of potassium glucose 6-sulfate prepared by direct sulphation of glucose and used as a substrate for the study of glycosulphatase were confirmed and extended. Potassium glucose 3-sulfate is readily hydrolyzed by glycosulphatase, but is unstable under the conditions necessary for determining sulfate by the benzidine method.