The survival of RP 1–1 inEscherichia coliand the influence of therecandpolAgenes on the process
- 1 August 1976
- journal article
- research article
- Published by Hindawi Limited in Genetics Research
- Vol. 28 (1), 15-26
- https://doi.org/10.1017/s0016672300016682
Abstract
The survival of the plasmid RP 1–1 in E. coli depends on the presence of a functional polA gene. Unlike other plasmids that have this requirement, the replication of RP 1–1 is inhibited by chloramphenicol. RP 1–1 cannot be inherited by E. coli recA mutants, and inactivation of the recA gene product in a recAts mutant leads to destruction of the plasmid. RP 1–1 cannot be inherited by recB or recC E. coli unless the strains also carry a suppressor of these genes, such as sbcA or sbcB. The pattern of replication of RP 1–1 in E. coli K12 and its mutants suggests that the survival of this plasmid in this species is the outcome of a balance between DNA polymerase I-specified replication and exonuclease destruction.This publication has 13 references indexed in Scilit:
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