Synthesis of a caged glutamate for efficient one- and two-photon photorelease on living cells
- 13 June 2005
- journal article
- Published by Royal Society of Chemistry (RSC) in Chemical Communications
- No. 29,p. 3664-3666
- https://doi.org/10.1039/b504922a
Abstract
We have synthesized a biologically inert, photosensitive derivative of the major excitatory amino acid, L-glutamate (which we call MDNI-glu) that makes more efficient use of incident light than all other caged glutamates. Laser flash photolysis of MDNI-glu in acutely isolated hippocampal brain slices evoked a rapid increase in intracellular Ca2+ concentration in astrocytes.Keywords
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