Effect of a large deletion of the basic domain of mitranscription factor on differentiation of mast cells
- 15 October 2001
- journal article
- Published by American Society of Hematology in Blood
- Vol. 98 (8), 2577-2579
- https://doi.org/10.1182/blood.v98.8.2577
Abstract
The mi transcription factor (MITF) is a basic-helix-loop-helix-leucine zipper transcription factor that is important for the development of mast cells. Cultured mast cells (CMCs) of mi/mi genotype express abnormal MITF (mi-MITF), but CMCs of tg/tg genotype do not express any MITFs. It was previously reported thatmi/mi CMCs showed more severe abnormalities thantg/tg CMCs, indicating that mi-MITF had inhibitory function. Whereas mi-MITF contains a single amino acid deletion in the basic domain, MITF encoded bymiewallele (ew-MITF) deletes 16 of 21 amino acids of the basic domain. Here the effect of a large deletion of the basic domain was examined. Inmiew/miew CMCs, the expression pattern of genes whose transcription was affected by MITF was comparable to that of tg/tg CMCs rather than to that ofmi/mi CMCs. This suggested that ew-MITF lacked any functions. The part of the basic domain deleted inew-MITF appeared necessary for either transactivation or inhibition of transactivation.Keywords
This publication has 16 references indexed in Scilit:
- Importance of leucine zipper domain of mitranscription factor (MITF) for differentiation of mast cells demonstrated using mice/mice mutant mice of which MITF lacks the zipper domainBlood, 2001
- Mutant Mice: A Useful Tool for Studying the Development of Mast CellsInternational Archives of Allergy and Immunology, 2001
- Inhibitory effect of the transcription factor encoded by the mutant mi microphthalmia allele on transactivation of mouse mast cell protease 7 geneBlood, 2001
- Insight into the microphthalmia geneTrends in Genetics, 1995
- microphthalmia, a critical factor in melanocyte development, defines a discrete transcription factor family.Genes & Development, 1994
- Molecular basis of mouse microphthalmia (mi) mutations helps explain their developmental and phenotypic consequencesNature Genetics, 1994
- Mutations at the mouse microphthalmia locus are associated with defects in a gene encoding a novel basic-helix-loop-helix-zipper proteinCell, 1993
- Heterogeneity of Mast Cells and Phenotypic Change Between SubpopulationsAnnual Review of Immunology, 1989
- Fate of bone marrow-derived cultured mast cells after intracutaneous, intraperitoneal, and intravenous transfer into genetically mast cell-deficient W/Wv mice. Evidence that cultured mast cells can give rise to both connective tissue type and mucosal mast cells.The Journal of Experimental Medicine, 1985
- The Coat Colors of MicePublished by Springer Nature ,1979