High-performance liquid-chromatographic determination theophylline in plasma.
- 1 February 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 24 (2), 357-360
- https://doi.org/10.1093/clinchem/24.2.357
Abstract
A high-performance liquid-chromatographic method is reported for monitoring theophylline in plasma. Samples are deproteinized with 2.5 volumes of acetonitrile and the supernates chromatographed on a reversed-phase column. Absorption at 275 nm is monitored. One can accurately measure 1.5 mg of theophylline per liter, in as little as 10 microliter of plasma, and only about 7 min is required per sample. No interference was found in plasma samples from asthmatic patients. This method is particularly useful for routine therapeutic monitoring of both pediatric and adult patients.This publication has 3 references indexed in Scilit:
- Rapid determination of theophylline in human plasma by high-pressure liquid chromatographyJournal of Pharmaceutical Sciences, 1976
- Determination of theophylline in plasma ultrafiltrate by reversed phase high pressure liquid chromatographyAnalytical Chemistry, 1976
- RAPID AND SENSITIVE METHOD FOR DETERMINATION OF THEOPHYLLINE IN PLASMA AND SALIVA BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY1976