Microscope Densitometer System for Point Measurement of Autoradiograms

Abstract

A photometric system for measuring optical densities generated by tissue antoradiograms has been adapted to an ordinary light microscope. The optics of the microscope are used to direct the light transmitted through the autoradiogram to ocular-mounted photoconductive cells coupled to a bridge amplifier. Readout is on a digital panel meter. The integrated area analyzed varies between 33.16 to 0.02 mm² depending on the objective magnification. The system is hear over a range of optical densities from 0.5 to 0.05.