Digestion of Collagen and Reticulin in Paraffin Sections by Collagenase
- 1 January 1960
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 35 (5), 273-276
- https://doi.org/10.3109/10520296009114748
Abstract
Tissues are fixed in ethanol or in Carnoy's 6:3:1 mixture and embedded in paraffin after routine ethanol dehydration. Sections are taken to water and then covered with 0.2 ml of a 0.9% NaCl solution containing 1 mg/ml of collagenase, and incubated at 50° C for 45 min. After this, they were washed and then stained by the usual methods for connective tissue fibers. Control sections were made by substituting plain 0.9% NaCl solution for the collagenase solution. The collagenase used was from bacteria and obtained from Nutritional Biochemicals Corporation, Cleveland 28, Ohio.Keywords
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