Quantitative High Pressure Liquid Chromatography of 6-Thioguanine in Biological Fluids
- 1 October 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 19 (10), 496-499
- https://doi.org/10.1093/chromsci/19.10.496
Abstract
The determination of 6-thioguanine in human plasma and urine is described. By using isocratic reversed-phase high pressure liquid chromatography (HPLC) the purine analogues, 6-thioguanine, guanine, 8-azaguanine, allopurinol, oxipurinol, and uric acid, are completely separated within 8 min. The sensitivity of the assay for 6-thioguanine is 0.2μg/ml (1.2 × 10-6M) with a precision of 3.7%. The applicability for clinical monitoring in a patient's plasma and urine is demonstrated.This publication has 8 references indexed in Scilit:
- The quantitative determination of metabolites of 6-mercaptopurine in biological materialsAnalytical Biochemistry, 1977
- Spectrofluorometric determination of thiopurines—I 6-ThioguanineTalanta, 1976
- BIOCHEMISTRY AND PHARMACOLOGY OF PURINE ANALOGUES1967
- BLOOD AND URINE LEVELS OF ANTITUMOR AGENTS DETERMINED WITH CELL CULTURE METHODS1965
- BASIC BIOCHEMICAL EFFECTS AND MECHANISM OF ACTION OF 6-THIOGUANINE1963
- A SUMMARY OF INVESTIGATIONS WITH 2-AMINO-6-[(1-METHYL-4-NITRO-5-IMIDAZOLYL)THIO]PURINE (BW-57-323) IN ANIMALS1960
- THE METABOLISM OF 2-AMINO-6-[(1-METHYL-4-NITRO-5-IMIDAZOLYL)THIO]PURINE (BW-57-323) IN MAN1960
- METABOLISM OF 6-THIOGUANINE IN NORMAL AND NEOPLASTIC TISSUES1958