The amino acid sequence of plastocyanin from spinach. (Spinacia oleracea L.)
- 1 May 1975
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 147 (2), 343-349
- https://doi.org/10.1042/bj1470343
Abstract
The amino acid sequence of spinach (Spinacia oleracea L.) plastocyanin was determined. It consists of a single polypeptide chain of 99 residues and has a sequence molecular weight of 10415. The sequence was determined by using a Beckman 890C automatic sequencer and by the dansyl--phenyl isothiocyanate analysis of peptides obtained by the enzymic digestion of purified CNBr fragments. Overlap through the two methionine residues was not shown. Sedimentation equilibrium in the ultracentrifuge gave a molecular weight for spinach plastocyanin of about 9000, in contrast with the value of 21000 reported previously by Katoh et al. (1962).Keywords
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