Parathyroid hormone stimulates prostanoid formation in mouse calvarial bones

Abstract

Bovine parathyroid hormone (bPTH 1–34) caused a time- and dose-dependent enhanced formation of the two prostanoids PGE₂ and 6-keto-PGF_1α in cultured neonatal mouse calvarial bones, with threshold for action at 0.1 nmol/l. The PGE₂ response to PTH was completely blocked by indomethacin, but insensitive to calcitonin. By contrast, indomethacin was without effect on ⁴⁵Ca release induced by PTH. The PTH analogues (Nle 8, 18, Tyr 34)-bPTH (3–34) amide and (Tyr 34)-bPTH (7–34) amide, which are putative PTH antagonists, did not affect basal production of PGE₂, nor did the analogues affect bPTH 1–34 induced PGE₂ formation. The data show that PTH stimulates prostanoid formation in mouse bone cells and that this response is not directly linked to PTH-induced bone resorption.