Idiotype positive T cells visualized by autoradiography and electron microscopy.
- 1 August 1975
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (8), 3210-3214
- https://doi.org/10.1073/pnas.72.8.3210
Abstract
Anti-idiotypic antibodies were prepared in (CBA X C57BL/6)F1 hybrid mice by immunization either with CBA anti-C57BL/6 alloantiserum or with purified CBA thymus-processed lymphoid cells (T cells) Iodinated anti-mouse Ig or triple sandwich ferritin-labeling techniques served to visualize the reaction between idiotype and anti-idiotype. From 5 to 10% of purified CBA T cells appeared to carry receptors for C57BL/6 antigens. Heavily labeled cells had the morphology of small lymphocytes.Keywords
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