INVITRO-PROPAGATION OF SEMINAL-VESICLE EPITHELIAL-CELLS

Abstract

The unique anatomic features of the guinea pig seminal vesicle allow establishment of nearly pure cultures of epithelial cells in vitro. Primary monolayer cultures can be maintained for up to 5 wk and suspension cultures for 5 to 7 days. The rapidly proliferating cells in monolayer culture are not markedly sensitive to the presence of androgens or estrogens in the medium. Scanning and transmission electron microscopy indicates that the proliferating cells are epithelial in nature and are quite different from control seminal vesicle fibroblast cultures; transmission electron microscopy shows that these cells are markedly dedifferentiated vis-a-vis the native tissue in vivo. This is the 1st report of the successful cultivation of seminal vesicle epithelial cells in vitro.