Incomplete flagellar structures in nonflagellate mutants of Salmonella typhimurium
- 1 February 1978
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 133 (2), 904-915
- https://doi.org/10.1128/jb.133.2.904-915.1978
Abstract
Incomplete flagellar structures were detected in osmotically shocked cells or membrane-associated fraction of many monflagellate mutants of S. typhimurium by EM. The predominant types of these structures in the mutants were cistron specific. The incomplete basal bodies were detected in flaFI, flaFIV, flaFVIII and flaFIX mutants, the structure homologous to a basal body in flaFV mutants, the polyhook-basal body complex in flaR mutants, and the hook-basal body complex in flaL and flaU mutants. No structures homologous to flagellar bases or their parts were detected in the early-fla group nonflagellate mutants of flaAI, flaAII, flaAIII, falB, flaC, flaD, flaE, flaFII, flaFIII, flaFVI, flaFVII, flaFX, flaK and flaM. From these observations, a process of flagellar morphogenesis was postulated. The functions of the early-fla group are essential to the formation of S ring-M ring-rod complexes bound to the membrane. The completion of basal bodies requires succeeding functions of flaFI, flaIV, flaFVIII and flaFIX. Next, the formation of hooks attached to basal bodies proceeds by the function of flaFV and by flaR, which controls the hook length. Flagellar filaments appear at the tips of hooks because of the functions of flaL, flaU and flagellin genes.This publication has 54 references indexed in Scilit:
- Sensitivity of Non-flagellate Salmonella Mutants to the Flagellotropic BacteriophageJournal of General Microbiology, 1977
- Morphogenesis of bacteriophage lambda tail. Polymorphism in the assembly of the major tail proteinJournal of Molecular Biology, 1976
- Genetic Analysis of H1, the Structural Gene for Phase-1 Flagellin in SalmonellaJournal of General Microbiology, 1975
- Absence of messenger ribonucleic acid specific for flagellin in non-flagellate mutants of SalmonellaJournal of Molecular Biology, 1975
- In vitro synthesis of phase-specific flagellin of SalmonellaJournal of Molecular Biology, 1973
- Possession of Flagellar Hooks by Some Non-flagellate Mutants of Salmonella abortusequiJournal of General Microbiology, 1972
- Genetic Analysis of fla and mot Cistrons Closely Linked to H1 in Salmonella abortusequi and its DerivativesJournal of General Microbiology, 1972
- Unidirectional growth of Salmonella flagella in vitroJournal of Molecular Biology, 1968
- Genetical Studies of Non-flagellate Mutants of SalmonellaJournal of General Microbiology, 1966
- A negative staining method for high resolution electron microscopy of virusesBiochimica et Biophysica Acta, 1959