Restriction and modification in Bacillus species
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 152 (1), 65-69
- https://doi.org/10.1007/bf00264941
Abstract
Host specific restriction was detected in 13 Bacillus strains, when 63 strains of Bacillus subtilis and 15 other Bacillus strains were tested with phage Φ105C. These 13 strains were classified into 8 groups (M,H,C,N,E,F,G,P) by the type of restriction. M-type strains (B. subtilis Marburg 168, its derivatives, and two other strains) showed relatively weak restriction, restricting Φ105C from other groups of Bacillus by ratios of 10-1 to 10-3. Strains of groups H,C,N,E,F,G, and P restricted Φ105C from other groups by ratios of 10-2 to 10-8. It was confirmed with some of the strains that type-specific modification was endowed only by the last host. Furthermore, we isolated one restriction deficient mutant of B. subtilis marburg 168-YS11, which had also lost its modification phenotype.Keywords
This publication has 13 references indexed in Scilit:
- The restriction endonucleases in Bacillus amyloliquefaciens N strain. Substrate specificitiesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
- Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens HJournal of Molecular Biology, 1975
- Host controlled modification and restriction in Bacillus subtilisMolecular Genetics and Genomics, 1974
- Restriction and modification in B. subtilisMolecular Genetics and Genomics, 1974
- A Suggested nomenclature for bacterial host modification and restriction systems and their enzymesJournal of Molecular Biology, 1973
- Circular chromosomal map of a temperate Bacillus phageVirology, 1967
- Bacteriophage Deoxyribonucleate Infection of Competent Bacillus subtilisJournal of Bacteriology, 1965