THE FLUORIMETRIC MICRODETERMINATION OF GLYOXYLIC ACID IN BLOOD, URINE AND BACTERIAL EXTRACTS
- 1 July 1965
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 96 (1), 218-223
- https://doi.org/10.1042/bj0960218
Abstract
A spectrophotofluorimetric method for the determination of glyoxylic acid in biological materials is described. The method is based on the reaction between glyoxylic acid and resorcinol in acid solution, a fluorescent complex being obtained on the subsequent addition of alkali. The reaction was found to be sensitive and highly specific, the minimum detectable amount of glyoxylic acid being 1-35 x 10-8 mole. The urinary excretion of glyoxylic acid by ten normal adults ranged from 1.4 to 4.7 mg/24 hr. Small but measurable amounts of glyoxylic acid were found in cell-free extracts of Pseudomonas oxalaticus OX1 grown on oxalic acid as a source of carbon. No glyoxylic acid was detected in human serum.Keywords
This publication has 13 references indexed in Scilit:
- PRIMARY HYPEROXALURIAMedicine, 1964
- GLYOXYLIC ACID CONTENT OF HUMAN BLOOD AND ITS RELATIONSHIP TO THIAMINE DEFICIENCY1963
- Studies on experimental thiamine deficiency. Trends of keto acid formation and detection of glyoxylic acidBiochemical Journal, 1962
- CONVERSION OFGAMMA-HYDROXYGLUTAMATE TO GLYOXYLATE AND ALANINE - PURIFICATIONAND PROPERTIES OF ENZYME SYSTEM1962
- Zur technik der papierchromatographischen trennung und bestimmung der α-ketosäuren im bluteClinica Chimica Acta; International Journal of Clinical Chemistry, 1961
- Carbon assimilation by Pseudomonas oxalaticus (OXI). 4. Metabolism of oxalate in cell-free extracts of the organism grown on oxalateBiochemical Journal, 1961
- Spectrophotometric measurement of α-keto acid semicarbazonesArchives of Biochemistry and Biophysics, 1959
- Les acides cétoniques du sang et de l'urine étude analytique; identification d'hydrazones nouvelles; applications cliniquesClinica Chimica Acta; International Journal of Clinical Chemistry, 1956
- The structure of ribonucleic acids. 2. The smaller products of ribonuclease digestionBiochemical Journal, 1952
- Studies in dialysis. 2. Changes of pH during dialysisBiochemical Journal, 1950