DNA polymorphisms amplified by arbitrary primers are useful as genetic markers
- 1 January 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 18 (22), 6531-6535
- https://doi.org/10.1093/nar/18.22.6531
Abstract
Molecular genetic maps are commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs) among the progeny of a sexual cross. Here we describe a new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. We suggest that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.This publication has 8 references indexed in Scilit:
- A Common Language for Physical Mapping of the Human GenomeScience, 1989
- DNA Diagnostics—Molecular Techniques and AutomationScience, 1988
- Simultaneous analysis of multiple polymorphic loci using amplified sequence polymorphisms (ASPs)Genomics, 1988
- Gene mapping with recombinant inbreds in maize.Genetics, 1988
- MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populationsGenomics, 1987
- CONSTRUCTION OF A GENETIC-LINKAGE MAP IN MAN USING RESTRICTION FRAGMENT LENGTH POLYMORPHISMS1980
- Rapid isolation of high molecular weight plant DNANucleic Acids Research, 1980