Ribonucleic Acid-Polyphosphate From Algae. I. Isolation & Physiology

Abstract
The blue-green alga Anabaena variabilis Kutz and the green alga Chlorella pyrenoidosa VN 2.2.1 were mass cultured for the isolation of RNA-poly-phosphate fractions. Purification consisted of solvent extractions and column chromatography. Within the total RNA-polyphosphate complex there was about 1 nucleotide/7 polyphosphate-P when the cultures were in a state of rapid growth. In synchronous Chlorella cultures significant changes were demonstrated to occur during the life cycle in both the absolute amounts and the relative proportions of RNA and polyphosphate of the various chromatographic subtractions obtained.