Identification of a unique isoform of 1-aminocyclopropane-1-carboxylic acid synthase by monoclonal antibody
- 1 December 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (23), 8810-8814
- https://doi.org/10.1073/pnas.85.23.8810
Abstract
1-aminocyclopropane-1-carboxylic acid (ACC) synthase (EC 4.41.14) is a key enzyme regulating ethylene biosynthesis in higher plants. A monoclonal antibody (mAb T20C) that immunoprecipitates the ACC synthase activity from tomato pericarp tissue extracts revealed that mAb T20C immunodecorates an .apprxeq.67-kDa polypeptide. On isoelectric focusing gels, ACC synthase activity in cell-free preparations was resolved into three distinct activity peaks with pI values 5.3, 7, and 9. mAb T20C specifically recognized the pI 7 form of the enzyme on electrophoretic transfer (Western) blots. When analyzed by sodium dodecyl sulfate gel electrophoresis under reducing conditions, the eluted pI 7 form was confirmed to migrate as a polypeptide of 67 kDa. The 67-kDa pI 7 isoform is a previously undescribed form of ACC synthase.Keywords
This publication has 16 references indexed in Scilit:
- Radiolabeling of a wound-inducible pyridoxal phosphate-utilizing enzyme: Evidence for its identification as ACC synthaseArchives of Biochemistry and Biophysics, 1987
- Use of monoclonal antibodies in the purification and characterization of 1-aminocyclopropane-1-carboxylate synthase, an enzyme in ethylene biosynthesisProceedings of the National Academy of Sciences, 1986
- Ethylene-regulated gene expression: molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris.Proceedings of the National Academy of Sciences, 1986
- A quantitative dot-immunobinding assay for proteins using nitrocellulose membrane filters.Proceedings of the National Academy of Sciences, 1984
- [9] Monoclonal antibodies against HLA products and their use in immunoaffinity purificationMethods in Enzymology, 1983
- Ultrasensitive Stain for Proteins in Polyacrylamide Gels Shows Regional Variation in Cerebrospinal Fluid ProteinsScience, 1981
- A simple and sensitive assay for 1-aminocyclopropane-1-carboxylic acidAnalytical Biochemistry, 1979
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Electrophoretic analysis of the major polypeptides of the human erythrocyte membraneBiochemistry, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970