Short-term, high-efficiency expression of transfected DNA.
Open Access
- 1 August 1984
- journal article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 4 (8), 1641-1643
- https://doi.org/10.1128/mcb.4.8.1641
Abstract
We have achieved high-efficiency uptake and expression of foreign DNA in mouse Ltk- cells by modifying the DEAE-dextran-mediated transfection method of McCutchan and Pagano (J. Natl. Cancer Inst. 42:351-357, 1968) to include an initial incubation at elevated pH followed by a shock treatment with dimethyl sulfoxide. Up to 80% of mouse Ltk- cells transfected with the herpes simplex virus thymidine kinase gene expressed thymidine kinase as measured by autoradiography.Keywords
This publication has 11 references indexed in Scilit:
- The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.Proceedings of the National Academy of Sciences, 1982
- Efficient DNA transfection and rapid assay for thymidine kinase activity and viral antigenic determinantsSomatic Cell and Molecular Genetics, 1982
- Efficient infection of monkey cells with DNA of simian virus 40.Proceedings of the National Academy of Sciences, 1981
- Mitogenic effect of alkaline pH on quiescent, serum-starved cells.Proceedings of the National Academy of Sciences, 1981
- SV40 DNA transfection of cells in suspension: analysis of the efficiency of transcription and translation of T-antigenGene, 1981
- Parameters governing the transfer of the genes for thymidine kinase and dihydrofolate reductase into mouse cells using metaphase chromosomes or DNASomatic Cell and Molecular Genetics, 1980
- A better cell line for making hybridomas secreting specific antibodiesNature, 1978
- Co-transfer of human X-linked markers into murine somatic cells via isolated metaphase chromosomesProceedings of the National Academy of Sciences, 1978
- A new technique for the assay of infectivity of human adenovirus 5 DNAVirology, 1973
- Enchancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran.1968