Temporal expression of membrane antigens during mouse spermatogenesis
Open Access
- 1 July 1977
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 74 (1), 86-97
- https://doi.org/10.1083/jcb.74.1.86
Abstract
Spermatogenic cells at advanced stages of differentiation, including pachytene primary spermatocytes, round spermatids and residual bodies of Regaud and mature spermatozoa, contain common antigenic membrane components which are not detected before the pachytene stage of the 1st meiotic prophase. These surface constituents are not detected on isolated populations of primitive type A spermatogonia, type A spermatogonia, type B spermatogonia, preleptotene primary spermatocytes or leptotene and zygotene primary spermatocytes. These results were demonstrated by immunofluorescence microscopy, by complement-mediated cytotoxicity, and by quantitative measurements of immunoglobulin (Ig) receptors on the plasma membranes of all cell populations examined. The cell surface antigens detected on germ cells are not found on mouse thymocytes, erythrocytes or peripheral blood lymphocytes as determined by immunofluorescence and by cytotoxicity assays. The absorption of antisera with kidney and liver tissue does not reduce the reactivity of the antibody preparations with spermatogenic cells, indicating that these antigenic determinants are specific to germ cells. This represents the 1st direct evidence for the ordered temporal appearance of plasma membrane antigens specific to particular classes of mouse spermatogenic cells. It appears that at late meiotic prophase, coincident with the production of pachytene primary spermatocytes, a variety of new components are inserted into the surface membranes of developing germ cells.This publication has 27 references indexed in Scilit:
- Spermatogenic cells of the prepuberal mouse: isolation and morphological characterizationThe Journal of cell biology, 1977
- THE TIME OF APPEARANCE OF Ia ANTIGENS DURING SPERMATOGENESIS IN THE MOUSETransplantation, 1976
- Developmental expression of an early embryonic antigen common to mouse spermatozoa and cleavage embryos, and to human spermatozoa: Its expression during spermatogenesisDevelopmental Biology, 1976
- The Sertoli cell occluding junctions and gap junctions in mature and developing mammalian testisDevelopmental Biology, 1976
- Effects of protease treatment on growth, morphology, adhesion, and cell surface proteins of secondary chick embryo fibroblastsCell, 1976
- Effect of proteases on activation of resting chick embryo fibroblasts and on cell surface proteinsCell, 1975
- TRANSCRIPTION SITES IN SPREAD MEIOTIC PROPHASE CHROMOSOMES FROM MOUSE SPERMATOCYTESThe Journal of cell biology, 1974
- The fine structure of the monkey (Macaca) sertoli cell and its role in maintaining the blood‐testis barrierThe Anatomical Record, 1973
- Morphogenetic factors influencing the shape of the sperm headDevelopmental Biology, 1971
- Synthetic activities during spermatogenesis in the mouseExperimental Cell Research, 1965