Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay
- 1 October 1994
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 32 (10), 2590-2
- https://doi.org/10.1128/jcm.32.10.2590-2592.1994
Abstract
A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylate. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.Keywords
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