The Use of Carbon Films in the Culture of Tissue Cells for Electron Microscopy
Open Access
- 25 March 1958
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 4 (2), 231-232
- https://doi.org/10.1083/jcb.4.2.231
Abstract
A technique is presented for propagating cells in tissue culture on carbon films which can subsequently be transferred to the electron microscope for observation of the undisturbed specimen. The chemically inert, non-toxic surface of the carbon is ideal for cell multiplication, has high stability under the electron beam and can be heat sterilized. Examples of pictures obtained by this technique are given.Keywords
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