The Use of Carbon Films in the Culture of Tissue Cells for Electron Microscopy

Abstract
A technique is presented for propagating cells in tissue culture on carbon films which can subsequently be transferred to the electron microscope for observation of the undisturbed specimen. The chemically inert, non-toxic surface of the carbon is ideal for cell multiplication, has high stability under the electron beam and can be heat sterilized. Examples of pictures obtained by this technique are given.