DEMONSTRATION OF BOVINE VIRAL DIARRHEA VIRUS ANTIGEN IN CRYOSTAT‐ AND PARAFFIN‐SECTIONS OF BOVINE TISSUES BY THE IMMUNOPEROXIDASE TECHNIQUE

Abstract

An immunoperoxidase technique (IPT) for the demonstration of bovine viral diarrhea virus (BVDV) antigen in tissue sections is presented. The method was applied to cryostat sections and to alcohol-fixed, paraffin-embedded specimens. The specificity as well as the sensitivity of the IPT corresponded to the immunofluorescence technique (IFT); the specimen processing methods used represented an improvement of the histomorphological preservation of tissue structures.